| Date | Home | Away | Result |
|---|---|---|---|
| 28/10/25 18:00 | CF Ourense | Real Oviedo | 4-2a.e.t |
| 28/10/25 18:00 | Atletico Tordesillas | Burgos CF | 1-2a.e.t |
| 28/10/25 18:00 | CE Constància | FC Girona | 2-3a.e.t |
| 28/10/25 18:30 | SD Logroñés | Racing Santander | 0-4 |
| 28/10/25 18:30 | UP Langreo | Racing de Ferrol | 3-4a.PSO |
| 28/10/25 18:30 | UCAM Murcia | FC Cadiz | 1-3 |
| 28/10/25 19:00 | CD Guadalajara | CP Cacereño | 2-1 |
| 28/10/25 19:00 | CF Rayo Majadahonda | CF Talavera de la Reina | 1-4 |
| 28/10/25 19:00 | UD San Fernando | Albacete Balompie | 0-3 |
| 28/10/25 19:00 | Inter de Valdemoro | FC Getafe | 0-11 |
| 28/10/25 19:00 | CD Roda | FC Granada | 1-5 |
| 28/10/25 19:00 | CD Tropezon | CyD Leonesa | 1-3 |
| 28/10/25 19:00 | UD Maracena | FC Valencia | 0-5 |
| 28/10/25 19:30 | Utebo | SD Huesca | 0-3 |
| 28/10/25 19:30 | RSD Alcalá | CD Teneriffa | 0-4 |
| 28/10/25 20:00 | CD Extremadura | UD Las Palmas | 3-1 |
| 28/10/25 20:00 | SD Negreira | Real Sociedad | 0-3 |
| 28/10/25 20:00 | CD Toledo | FC Sevilla | 1-4 |
| 29/10/25 17:00 | UD Poblense | CD Sabadell | --- |
| 29/10/25 18:00 | CD Ebro | SD Tarazona | --- |
| 29/10/25 18:00 | Union Mutilvera | Real Saragossa | --- |
| 29/10/25 18:00 | CD Sant Jordi | CA Osasuna | --- |
| 29/10/25 18:00 | Los Garres | FC Elche | --- |
| 29/10/25 18:30 | La Unión Atlético CF | AD Ceuta FC | --- |
| 29/10/25 19:00 | CD Artistico Navalcarnero | Merida AD | --- |
| 29/10/25 19:00 | CD Azuaga | CD Leganes | --- |
| 29/10/25 19:00 | Náxara CD | SD Eibar | --- |
| 29/10/25 19:00 | Atletico Astorga | CD Mirandes | --- |
| 29/10/25 19:00 | Club Portugalete | Real Valladolid | --- |
| 29/10/25 19:00 | CD Numancia | Arenas Club de Getxo | --- |
| 29/10/25 19:00 | Lorca Deportiva CF | UD Almeria | --- |
| 29/10/25 19:00 | UE Sant Andreu | CD Teruel | --- |
| 29/10/25 19:00 | Caudal Deportivo | Sporting Gijon | --- |
| 29/10/25 19:00 | Atlètic Sant Just | RCD Mallorca | --- |
| 29/10/25 19:00 | Torrent CF | Juventud Torremolinos CF | --- |
| 29/10/25 19:00 | CD Cieza | Cordoba CF | --- |
| 29/10/25 19:30 | CF Reus Deportiu | CE Europa | --- |
| 29/10/25 19:30 | UD Ourense | CF Pontevedra | --- |
| 29/10/25 19:30 | Puente Genil CF | FC Cartagena | --- |
| 29/10/25 20:00 | CD Quintanar del Rey | UD Ibiza Eivissa | --- |
| 29/10/25 20:00 | Real Jaén C.F. | CD Eldense | --- |
| 29/10/25 20:00 | CF Lucena | FC Villarreal | --- |
| 29/10/25 20:00 | CD Yuncos | Rayo Vallecano | --- |
| 30/10/25 18:00 | Orihuela CF | UD Levante | --- |
| 30/10/25 18:00 | Puerto de Vega CF | Celta Vigo | --- |
| 30/10/25 18:30 | Valle de Egues | Andorra CF | --- |
| 30/10/25 19:00 | Atletico Baleares | Gimnastic de Tarragona | --- |
| 30/10/25 19:00 | UD Logroñés | SD Ponferradina | --- |
| 30/10/25 19:00 | Real Ávila CF | Real Avilés | --- |
| 30/10/25 19:00 | CA Antoniano | CD Castellon | --- |
| 30/10/25 19:00 | Arenas Club de Getxo | Deportivo Alaves | --- |
| 30/10/25 20:00 | Real Murcia C. F. | Antequera CF | --- |
| 30/10/25 20:00 | CD Estepona | FC Malaga | --- |
| 30/10/25 20:00 | UD Samano | Deportivo La Coruna | --- |
| 30/10/25 20:00 | Club Lleida Esportiu | Espanyol Barcelona | --- |
| 30/10/25 20:00 | Atletico Palma del Rio | Real Betis Sevilla | --- |