| Date | Home | Away | Result |
|---|---|---|---|
| 27/10/26 17:30 | CD Cuarte | FC Girona | 0-2 |
| 27/10/26 18:00 | Sestao River Club | Espanyol Barcelona | 4-3a.e.t |
| 27/10/26 18:00 | CF Calamocha | CE Europa | 0-3 |
| 27/10/26 18:00 | Atletico Pinatarense | FC Elche | 1-3 |
| 27/10/26 18:45 | UD Castellonense | UD Almeria | 1-2a.e.t |
| 27/10/26 19:00 | Atletico Tordesillas | CD Mirandes | 5-7a.PSO |
| 27/10/26 19:00 | CF Lucena | CF Rayo Majadahonda | 0-1 |
| 27/10/26 19:00 | Gimnastica Segoviana | CyD Leonesa | 0-1 |
| 27/10/26 19:00 | UD Llanera | SD Ponferradina | 4-6a.PSO |
| 27/10/26 19:00 | RSD Alcalá | UD Las Palmas | 1-3a.e.t |
| 27/10/26 19:30 | CD Don Benito | CD Extremadura | 2-4a.e.t |
| 27/10/26 19:30 | CE Manresa | UE Sant Andreu | 3-6a.PSO |
| 27/10/26 20:00 | Arosa SC | CF Pontevedra | 2-0 |
| 27/10/26 20:00 | CD Atletico Paso | Cordoba CF | 3-1 |
| 27/10/26 20:00 | CD Tudelano | Real Saragossa | 0-4 |
| 27/10/26 20:00 | CD Manacor | FC Villarreal | 0-5 |
| 27/10/26 20:00 | Recreativo Huelva | Rayo Vallecano | 2-3a.e.t |
| 27/10/26 20:00 | Sporting de Alcazar | FC Malaga | 0-3 |
| Cancelled | CD Anaitasuna | Deportivo Alaves | --- |
| Cancelled | Utebo | CD Sabadell | --- |
| Cancelled | FC Coruxo | Zamora CF | --- |
| Cancelled | FC Pena Sport | SD Huesca | --- |
| Cancelled | Club Portugalete | Real Union Club | --- |
| Cancelled | UD Poblense | AD Ceuta FC | --- |
| Cancelled | CD Tarancon | UD Levante | --- |
| Cancelled | CD Pamplona | SD Eibar | --- |
| Cancelled | SD Amorebieta | UD Logroñés | --- |
| Cancelled | CD Alcoyano | RCD Mallorca | --- |
| Cancelled | CD Badajoz | CD Teneriffa | --- |
| Cancelled | CD Covadonga | Real Valladolid | --- |
| Cancelled | CD Numancia | Real Oviedo | --- |
| Cancelled | CS Puertollano | Albacete Balompie | --- |
| Cancelled | Trival Valderas | CD Leganes | --- |
| Cancelled | Mijas-Las Lagunas | Real Jaén C.F. | --- |
| Cancelled | CD Cieza | FC Granada | --- |
| Cancelled | Deportiva Minera | FC Cartagena | --- |
| Cancelled | CF Reus Deportiu | Barakaldo CF | --- |
| Cancelled | RS Gimnastica | UD Ourense | --- |
| Cancelled | Salamanca CF UDS | Celta Vigo | --- |
| Cancelled | Deportivo Xerez | FC Cadiz | --- |
| Cancelled | CD Artistico Navalcarnero | Real Betis Sevilla | --- |
| Cancelled | Tavernes de la Valldigna | FC Valencia | --- |
| 29/10/26 18:00 | Atletico Baleares | CD Eldense | --- |
| 29/10/26 18:00 | San Sebastian de los Reyes | CD Coria | --- |
| 29/10/26 18:00 | Atletico Calatayud | CA Osasuna | --- |
| 29/10/26 18:00 | UB Lebrijana | FC Getafe | --- |
| 29/10/26 19:00 | Antequera CF | CDA Aguilas FC | --- |
| 29/10/26 19:00 | CD Varea | Andorra CF | --- |
| 29/10/26 19:00 | Ub Conquense | CD Castellon | --- |
| 29/10/26 19:00 | CD Laredo | Burgos CF | --- |
| 29/10/26 19:00 | CD Guijuelo | Sporting Gijon | --- |
| 29/10/26 19:00 | Real Ávila CF | Unionistas de Salamanca | --- |
| 29/10/26 20:00 | Auriense CA | Deportivo La Coruna | --- |
| 29/10/26 20:00 | Ribadesella CF | Racing Santander | --- |
| 29/10/26 20:00 | Sporting Hortaleza | FC Sevilla | --- |
| 29/10/26 20:00 | AE Prat | Athletic Bilbao | --- |